axion vert a1 inverted microscope  (Carl Zeiss)

Journal: Redox Biology

Article Title: Multi-organ toxicity via oxidative stress and disrupting mitochondrial plasticity induced by bendiocarb in zebrafish

doi: 10.1016/j.redox.2025.104001

Figure Lengend Snippet: Bendiocarb-triggered apoptosis in zebrafish. [A and B] Acridine orange was used to stain the bendiocarb (0, 1, 2, and 4 mg/L)-treated groups (n = 15 per group), and the fluorescence measured. Scale bar: 100 μm [C–E] mRNA expression (n = 25 per group) of genes related to apoptosis ( casp9 , bcl2 , and bcl-xl ) measured using qRT-PCR. [F and G] TUNEL assay was performed (n = 15 per group), and fluorescent images were obtained using the LSM700 confocal microscope. The number of TUNEL-positive cells was quantified using ImageJ software and presented as a graph. Statistical significance compared with the vehicle group (0 mg/L bendiocarb) is indicated by asterisks (∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001). Differences among groups were assessed using one-way ANOVA followed by Dunnett's post-hoc test comparing each treatment group to the control. TUNEL assay were analyzed using Student's t-test. All experiments were performed in triplicate.

Article Snippet: Fluorescence signals were visualized using an inverted fluorescence microscope (Axio Vert.A1 FL; ZEISS, Oberkochen, Germany) equipped with a GFP filter.

Techniques: Staining, Fluorescence, Expressing, Quantitative RT-PCR, TUNEL Assay, Microscopy, Software, Control